You can duplicate hard disk image files on the same host to
quickly produce a second virtual machine with the same OS setup.
However, you should only make copies of
virtual disk images using the utility supplied with
Oracle VM VirtualBox. See
Note that Linux distributions identify the boot hard disk from the
ID of the drive. The ID Oracle VM VirtualBox reports for a drive is
determined from the UUID of the virtual disk image. So if you
clone a disk image and try to boot the copied image the guest
might not be able to determine its own boot disk as the UUID
changed. In this case you have to adapt the disk ID in your boot
loader script, for example
scsi-SATA_VBOX_HARDDISK_VB5cfdb1e2-c251e503
The ID for the copied image can be determined as follows:
hdparm -i /dev/sda